Utiliza este identificador para citar o vincular este elemento: http://hdl.handle.net/10553/35367
Títulos: Skeletal muscle signaling, metabolism, and performance during sprint exercise in severe acute hypoxia after the ingestion of antioxidants
Autores/as: Morales-Alamo, David 
Guerra, Borja 
Ponce-González, Jesús Gustavo
Guadalupe Grau, Amelia 
Santana Rodríguez, Alfredo 
Martín Rincón, Marcos 
Gelabert-Rebato, Miriam 
Cadefau, Joan A.
Cusso, Roser
Dorado, C 
Calbet, José A.L. 
Clasificación UNESCO: 241106 Fisiología del ejercicio
Palabras clave: Sprint exercise
Skeletal muscle
Hypoxia
Human
Adenosine5'-monophosphate-activated protein kinase
Oxidative stress
Fecha de publicación: 2017
Revistas: Journal of Applied Physiology 
Resumen: The aim of this study was to determine if reactive oxygen species (ROS) could play a role in blunting Thr172-AMP-activated protein kinase (AMPK)-α phosphorylation in human skeletal muscle after sprint exercise in hypoxia and to elucidate the potential signaling mechanisms responsible for this response. Nine volunteers performed a single 30-s sprint (Wingate test) in two occasions while breathing hypoxic gas (PIO2 = 75 mmHg): one after the ingestion of placebo and another following the intake of antioxidants (-lipoic acid, vitamin C, and vitamin E), with a randomized double-blind design. Vastus lateralis muscle biopsies were obtained before, immediately after, and 30- and 120-min postsprint. Compared with the control condition, the ingestion of antioxidants resulted in lower plasma carbonylated proteins, attenuated elevation of the AMP-to-ATP molar ratio, and reduced glycolytic rate (P < 0.05) without significant effects on performance or V ? O2. The ingestion of antioxidants did not alter the basal muscle signaling. Thr172-AMPK- and Thr184/187-transforming growth factor-activated kinase 1 (TAK1) phosphorylation were not increased after the sprint regardless of the ingestion of antioxidants. Thr286-CaMKII phosphorylation was increased after the sprint, but this response was blunted by the antioxidants. Ser485-AMPKα1/Ser491-AMPKα2 phosphorylation increased immediately after the sprints coincident with increased Akt phosphorylation. In summary, antioxidants attenuate the glycolytic response to sprint exercise in severe acute hypoxia and modify the muscle signaling response to exercise. Ser485-AMPK α 1/Ser491-AMPKα2 phosphorylation, a known mechanism of Thr172- AMPKα phosphorylation inhibition, is increased immediately after sprint exercise in hypoxia, probably by a mechanism independent of ROS.
URI: http://hdl.handle.net/10553/35367
ISSN: 8750-7587
DOI: 10.1152/japplphysiol.00384.2017
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