Utiliza este identificador para citar o vincular este elemento: http://hdl.handle.net/10553/1729
Títulos: Sporulation and sterilization method for axenic culture of Gelidium canariensis
Autores/as: García-Jiménez, Pilar 
Marián de Diego, Fernando
Rodrigo Sanz, Marta
Robaina Romero, Rafael 
Clasificación UNESCO: Algas rojas
Algas marinas
Cultivo
Rhodophyta
Fecha de publicación: 1999
Resumen: A sporulation and sterilization procedure was used to establish axenic cultures of sporelings of Gelidium canariensis. Sporangial branchlets excised from the thallus were rinsed in distilled water twice and in 1% sodium hypochlorite (2min). The branchlets were cultivated overnight in multiwell plates with 0.3 ml of autoclaved seawater to promote spore liberation in 90% of the cultivated branchlets. The branchlets were transferred to an antibiotic solution made of ampicillin, penicillin, rifampicin, nystatin (0.2 mg ml-1 each) and 0.1 g ml-1 of GeO2 in liquid PES for 45 days, during which clusters of spores (85 – 100 spores) were observed on the surface of the branchlet. After 55 days, they became axenic sporelings with the prostrate and erect system characteristic of Gelidium canariensis.
URI: http://hdl.handle.net/10553/1729
ISSN: 0168-1656
DOI: 10.1016/S0168-1656(99)00077-2
Aparece en la colección:Artículos

Muestra el registro completo del elemento

Citas SCOPUSTM   

8
actualizado el 26-nov-2018

Vista de página(s)

9
actualizado el 21-ene-2019

Google ScholarTM

Verifica

Altmetric


Los elementos en ULPGC accedaCRIS están protegidos por derechos de autor, con todos los derechos reservados, a menos que se indique lo contrario.